A novel chimaeric flocculation protein enhances flocculation in Saccharomyces cerevisiae
نویسندگان
چکیده
منابع مشابه
Flocculation onset, growth phase, and genealogical age in Saccharomyces cerevisiae.
Flocculation onset, the time during the fermentative cycle at which the strains of Saccharomyces cerevisiae become flocculent, is an important factor in the brewing industry. The flocculation ability of Flo 1 phenotype (strain NCYC 869) remained practically unchanged throughout the growth and seems to be insensitive to the presence of nutrients of the culture medium. On the contrary, the floccu...
متن کاملLocalization and cell surface anchoring of the Saccharomyces cerevisiae flocculation protein Flo1p.
The Saccharomyces cerevisiae FLO1 gene encodes a large 1,536-amino-acid serine- and threonine-rich protein involved in flocculation. We have assessed the localization of Flo1p by immunoelectron microscopy, and in this study we show that this protein is located in the external mannoprotein layer of the cell wall, at the plasma membrane level and in the periplasm. The protein was also visualized ...
متن کاملFlocculation causes inhibitor tolerance in Saccharomyces cerevisiae for second-generation bioethanol production.
Yeast has long been considered the microorganism of choice for second-generation bioethanol production due to its fermentative capacity and ethanol tolerance. However, tolerance toward inhibitors derived from lignocellulosic materials is still an issue. Flocculating yeast strains often perform relatively well in inhibitory media, but inhibitor tolerance has never been clearly linked to the actu...
متن کاملFlocculation onset in Saccharomyces cerevisiae: effect of ethanol, heat and osmotic stress.
AIMS To examine the effect of different stress conditions on the onset of flocculation in an ale-brewing strain, Saccharomyces cerevisiae NCYC 1195. METHODS AND RESULTS Flocculation was evaluated using the method of Soares, E.V. and Vroman, A. [Journal of Applied Microbiology (2003) 95, 325]; plasma membrane integrity was accessed using propidium iodide and the staining of the yeast cell wall...
متن کاملControlled expression of the dominant flocculation genes FLO1, FLO5, and FLO11 in Saccharomyces cerevisiae.
In many industrial fermentation processes, the Saccharomyces cerevisiae yeast should ideally meet two partially conflicting demands. During fermentation, a high suspended yeast count is required to maintain a satisfactory rate of fermentation, while at completion, efficient settling is desired to enhance product clarification and recovery. In most fermentation industries, currently used starter...
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ژورنال
عنوان ژورنال: Metabolic Engineering Communications
سال: 2018
ISSN: 2214-0301
DOI: 10.1016/j.meteno.2018.04.001